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Lookup NU author(s): James Connolly
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2016 Connolly et al. The ability of any organism to sense and respond to challenges presented in the environment is critically important for promoting or restricting colonization of specific sites. Recent work has demonstrated that the host metabolite D-serine has the ability to markedly influence the outcome of infection by repressing the type III secretion system of enterohaemorrhagic Escherichia coli (EHEC) in a concentration-dependent manner. However, exactly how EHEC monitors environmental D-serine is not understood. In this work, we have identified two highly conserved members of the E. coli core genome, encoding an inner membrane transporter and a transcriptional regulator, which collectively help to “sense” levels of D-serine by regulating its uptake from the environment and in turn influencing global gene expression. Both proteins are required for full expression of the type III secretion system and diversely regulated prophage-encoded effector proteins demonstrating an important infection-relevant adaptation of the core genome. We propose that this system acts as a key safety net, sampling the environment for this metabolite, thereby promoting colonization of EHEC to favorable sites within the host.
Author(s): Connolly JPR, Gabrielsen M, Goldstone RJ, Grinter R, Wang D, Cogdell RJ, Walker D, Smith DGE, Roe AJ
Publication type: Article
Publication status: Published
Journal: PLoS Pathogens
Online publication date: 04/01/2016
Acceptance date: 03/12/2015
Date deposited: 20/09/2019
ISSN (print): 1553-7366
ISSN (electronic): 1553-7374
Publisher: Public Library of Science
PubMed id: 26727373
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