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Genomic sequence and host range studies reveal considerable variation within the species Arracacha virus B

Lookup NU author(s): Professor Neil Boonham, Dr Ian Adams

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer, 2019.

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Abstract

Arracacha virus B type (AVB-T) and oca (AVB-O) strains from arracacha (Arracacia xanthorrhiza) and oca (Oxalis tuberosa) samples collected in 1975 and two additional isolates obtained from arracacha (AVB-PX) and potato (AVB-6A) in Peru in 1976 and 1978, respectively, were studied. In its host responses and serological properties, AVB-PX most resembled AVB-T, whereas AVB-6A most resembled AVB-O. Complete genomic sequences of the RNA-1 and RNA-2 of each isolate were obtained following high-throughput sequencing of RNA extracts from isolates preserved for 38 (AVB-PX) or 32 (the other 3 isolates) years, and compared with a genomic sequence of AVB-O obtained previously (PV-0082). RNA-2 was unexpectedly divergent compared to RNA-1, with the nucleotide (nt) sequence identity of different AVB isolates varying by up to 76% (RNA-2) and 89% (RNA-1). The coat protein amino acid sequences were the most divergent, with AVB-O and AVB-6A having only 68% identity to AVB-T and AVB-PX. Since the RNA2 sequence differences between the two isolate groupings also coincided with host range, symptom, and serological differences, AVB demonstrates considerable intraspecific divergence.


Publication metadata

Author(s): Jones RAC, Fox A, Boonham N, Adams IP

Publication type: Article

Publication status: Published

Journal: Archives of Virology

Year: 2019

Volume: 164

Issue: 11

Pages: 2849-2852

Print publication date: 01/11/2019

Online publication date: 09/09/2019

Acceptance date: 05/08/2019

Date deposited: 07/11/2019

ISSN (print): 0304-8608

ISSN (electronic): 1432-8798

Publisher: Springer

URL: https://doi.org/10.1007/s00705-019-04395-7

DOI: 10.1007/s00705-019-04395-7

PubMed id: 31502078


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