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Lookup NU author(s): Dr Valeria Chichagova, Dr Gerrit HilgenORCiD, Dr Ali Ghareeb, Madeleine Carter, Professor Evelyne SernagorORCiD, Professor Majlinda LakoORCiD, Professor Lyle Armstrong
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Induced pluripotent stem cell (iPSC)-derived retinal organoids provide a platform to study human retinogenesis, disease modelling and compound screening. Whilst retinal organoids may represent tissue structures with greater physiological relevance to the in vivo human retina, their generation is not without limitations. Various protocols have been developed to enable development of organoids with all major retinal cell types, however variability across iPSC lines is often reported. Modulating signalling pathways important for eye formation, such as those involving bone morphogenetic protein 4 (BMP4) and insulin-like growth factor (IGF1), is a common approach used for the generation of retinal tissue in vitro. We used three human iPSC lines to generate retinal organoids by activating either BMP4 or IGF1 signalling and assessed differentiation efficiency by monitoring morphological changes, gene and protein expression, and function. Our results showed that iPSC ability to give rise to retinal organoids in response to IGF1 and BMP4 activation was line- and method-dependent. This demonstrates that careful consideration is needed when choosing a differentiation approach, which would also depend on overall project aims.
Author(s): Chichagova V, Hilgen G, Ghareeb A, Gergiou M, Carter M, Sernagor E, Lako M, Armstrong L
Publication type: Article
Publication status: Published
Journal: Stem Cells
Year: 2020
Volume: 38
Issue: 2
Pages: 195-201
Print publication date: 01/02/2020
Online publication date: 13/11/2019
Acceptance date: 01/11/2019
Date deposited: 07/11/2019
ISSN (print): 1066-5099
ISSN (electronic): 1549-4918
Publisher: AlphaMed Press, Inc.
URL: https://doi.org/10.1002/stem.3116
DOI: 10.1002/stem.3116
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