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Lookup NU author(s): Dr James Knight
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Site-selective labelling of antibodies (Abs) can circumvent problemsfrom heterogeneity of conventional conjugation. Here, we evaluatethe industrially-applied chemoenzymatic ‘Q-tag’ strategy based ontransglutaminase-mediated (TGase) amide-bond formation in thegeneration of 89Zr-radiolabelled antibody conjugates. We showthat, despite previously suggested high regioselectivity of TGases,in the anti-Her2 Ab Herceptint more precise native MS indicatesonly 70–80% functionalization at the target site (Q298H), in competitionwith modification at other sites, such as Q3H critically close to theCDR1 region.
Author(s): Marculescu C, Lakshminarayanan A, Gault J, Knight JC, Folkes LK, Spink T, Robinson CV, Valis K, Davis BG, Cornelissen B
Publication type: Article
Publication status: Published
Journal: Chemical Communications
Print publication date: 03/09/2019
Online publication date: 03/09/2019
Acceptance date: 25/07/2019
Date deposited: 17/12/2019
ISSN (print): 1359-7345
ISSN (electronic): 1364-548X
Publisher: Roal Society of Chemistry
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