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A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications

Lookup NU author(s): Dr Alexander Sieberath, Dr Ana Ferreira-DuarteORCiD, Dr Piergiorgio GentileORCiD, Professor Kenneth Dalgarno



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


As the population of western societies on average ages, the number of people affected by bone remodeling-associated diseases such as osteoporosis continues to increase. The development of new therapeutics is hampered by the high failure rates of drug candidates during clinical testing, which is in part due to the poor predictive character of animal models during preclinical drug testing. Co-culture models of osteoblasts and osteoclasts offer an alternative to animal testing and are considered to have the potential to improve drug development processes in the future. However, a robust, scalable, and reproducible 3D model combining osteoblasts and osteoclasts for preclinical drug testing purposes has not been developed to date. Here we review various types of osteoblast-osteoclast co-culture models and outline the remaining obstacles that must be overcome for their successful translation.

Publication metadata

Author(s): Sieberath A, Della Bella E, Ferreira AM, Gentile P, Eglin D, Dalgarno K

Publication type: Review

Publication status: Published

Journal: International Journal of Molecular Sciences

Year: 2020

Volume: 21

Issue: 3

Online publication date: 30/01/2020

Acceptance date: 21/01/2020

ISSN (print): 1661-6596

ISSN (electronic): 1422-0067

Publisher: NLM (Medline)


DOI: 10.3390/ijms21030912

PubMed id: 32019244