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Lookup NU author(s): Dr Rachel Botting, Dr Cecilia Dominguez Conde, Dr Dorin-Mirel Popescu, Dr Emily StephensonORCiD, Dr Simone Webb, Dan Maunder, Dr David Crossland, Andrew Fuller, Professor Andrew FilbyORCiD, Dr Gary ReynoldsORCiD, David Dixon, Dr Steven LisgoORCiD, Professor Deborah HendersonORCiD, Dr Roser Vento-Tormo, Professor Muzlifah Haniffa
This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Assocaition for the Advancement of Science (AAAS), 2020.
For re-use rights please refer to the publisher's terms and conditions.
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.The thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We used single-cell RNA sequencing to create a cell census of the human thymus across the life span and to reconstruct T cell differentiation trajectories and T cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ CD8αα+ T cell populations, thymic fibroblast subtypes, and activated dendritic cell states. In addition, we reveal a bias in TCR recombination and selection, which is attributed to genomic position and the kinetics of lineage commitment. Taken together, our data provide a comprehensive atlas of the human thymus across the life span with new insights into human T cell development.
Author(s): Park J-E, Botting RA, Dominguez Conde C, Popescu D-M, Lavaert M, Kunz DJ, Goh I, Stephenson E, Ragazzini R, Tuck E, Wilbrey-Clark A, Roberts K, Kedlian VR, Ferdinand JR, He X, Webb S, Maunder D, Vandamme N, Mahbubani KT, Polanski K, Mamanova L, Bolt L, Crossland D, de Rita F, Fuller A, Filby A, Reynolds G, Dixon D, Saeb-Parsy K, Lisgo S, Henderson D, Vento-Tormo R, Bayraktar OA, Barker RA, Meyer KB, Saeys Y, Bonfanti P, Behjati S, Clatworthy MR, Taghon T, Haniffa M, Teichmann SA
Publication type: Article
Publication status: Published
Journal: Science
Year: 2020
Volume: 367
Issue: 6480
Print publication date: 21/02/2020
Online publication date: 21/02/2020
Acceptance date: 16/01/2020
Date deposited: 12/03/2020
ISSN (print): 0036-8075
ISSN (electronic): 1095-9203
Publisher: American Assocaition for the Advancement of Science (AAAS)
URL: https://doi.org/10.1126/science.aay3224
DOI: 10.1126/science.aay3224
PubMed id: 32079746
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