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Propagation of human prostate tissue from induced pluripotent stem cells

Lookup NU author(s): Dr Anastasia Hepburn, Emma Curry, Dr Mohammad Moad, Laura Wilson, Parmveer Singh, Dr Adriana Buskin, Dr Luke Gaughan, Professor Craig Robson, Professor Rakesh Heer

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2020 The Authors. STEM CELLS TRANSLATIONAL MEDICINE published by Wiley Periodicals, Inc. on behalf of AlphaMed Press. Primary culture of human prostate organoids and patient-derived xenografts is inefficient and has limited access to clinical tissues. This hampers their use for translational study to identify new treatments. To overcome this, we established a complimentary approach where rapidly proliferating and easily handled induced pluripotent stem cells enabled the generation of human prostate tissue in vivo and in vitro. By using a coculture technique with inductive urogenital sinus mesenchyme, we comprehensively recapitulated in situ 3D prostate histology, and overcame limitations in the primary culture of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. This model now unlocks new opportunities to undertake translational studies of benign and malignant prostate disease.


Publication metadata

Author(s): Hepburn AC, Curry EL, Moad M, Steele RE, Franco OE, Wilson L, Singh P, Buskin A, Crawford SE, Gaughan L, Mills IG, Hayward SW, Robson CN, Heer R

Publication type: Article

Publication status: Published

Journal: Stem Cells Translational Medicine

Year: 2020

Volume: 9

Issue: 7

Pages: 734-745

Print publication date: 01/07/2020

Online publication date: 14/03/2020

Acceptance date: 29/01/2020

Date deposited: 18/05/2021

ISSN (print): 2157-6564

ISSN (electronic): 2157-6580

Publisher: John Wiley and Sons Ltd

URL: https://doi.org/10.1002/sctm.19-0286

DOI: 10.1002/sctm.19-0286


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