Toggle Main Menu Toggle Search

Open Access padlockePrints

Characterization of a gene encoding a single-subunit bacteriophage-type RNA polymerase from maize which is alternatively spliced

Lookup NU author(s): Professor David YoungORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Single-subunit RNA polymerases belonging to the T3/T7 bacteriophage family are thought to be common throughout eukaryotes. We report the isolation and characterization of a nucleus-encoded single-subunit RNA polymerase gene from maize. This gene is highly homologous to other single-subunit RNA polymerase genes from Arabidopsis, Chenopodium. yeast and Neurospora crassa involved in organellar transcription. Genomic Southern analysis reveals 10 to 15 hybridising fragments, suggesting that maize contains a small gene family. The isolated gene contains 19 exons and its genomic structure is highly conserved when compared to the three Arabidopsis homologues. Unlike the case in Arabidopsis, intron-12 of the maize bacteriophage-type RNA polymerase gene is alternatively spliced. Quantitative RT-PCR revealed that the resultant alternatively spliced transcript represents approximately 21 to 26% of the total polymerase mRNA in maize coleoptiles. The orthologous wheat bacteriophage-type RNA polymerase is also alternatively spliced and the intron exhibits 78% identity to maize intron-12. The conservation in alternative splicing between wheat and maize and its absence from Arabidopsis suggest a functional requirement for the alternatively spliced product.

Publication metadata

Author(s): Young DA, Allen RL, Harvey AJ, Lonsdale DM

Publication type: Article

Publication status: Published

Journal: Molecular & General Genetics

Year: 1998

Volume: 260

Issue: 1

Pages: 30-7

Print publication date: 01/10/1998

ISSN (print): 0026-8925

ISSN (electronic): 1617-4623

Publisher: Springer