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A rule-based data-informed cellular consensus map of the human mononuclear phagocyte cell space

Lookup NU author(s): Professor Venetia BigleyORCiD, Professor Matthew CollinORCiD

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).

Abstract

Single-cell genomic techniques are opening new avenues to understand the basic units of life. Large international efforts, such as those to derive a Human Cell Atlas, are driving progress in this area; here, cellular map generation is key. To expedite the inevitable iterations of these underlying maps, we have developed a rule-based data-informed approach to build next generation cellular consensus maps. Using the human dendritic-cell and monocyte compartment in peripheral blood as an example, we performed computational integration of previous, partially overlapping maps using an approach we termed ‘backmapping’, combined with multi-color flow- cytometry and index sorting-based single-cell RNA-sequencing. Our general strategy can be applied to any atlas generation for humans and other species.

Publication metadata

Author(s): Günther P, Cirovic B, Bassler K, Händler K, Becker M, Dutertre CA, Bigley V, Newell E, Collin M, Ginhoux F, Schlitzer A, Schultze JL

Publication type: Article

Publication status: Published

Journal: bioRxiv

Year: 2019

Pages: 1-59

Online publication date: 03/06/2019

Acceptance date: 03/06/2019

Date deposited: 09/07/2020

ISSN (electronic): 2692-8205

Publisher: bioRxiv

URL: https://doi.org/10.1101/658179

DOI: 10.1101/658179

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