Toggle Main Menu Toggle Search

Open Access padlockePrints

A pond-side test for Guinea worm: Development of a loop-mediated isothermal amplification (LAMP) assay for detection of Dracunculus medinensis

Lookup NU author(s): Professor Neil Boonham



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2020 The AuthorsGuinea worm Dracunculus medinensis causes debilitating disease in people and is subject to an ongoing global eradication programme. Research and controls are constrained by a lack of diagnostic tools. We developed a specific and sensitive LAMP method for detecting D. medinensis larval DNA in copepod vectors. We were able to detect a single larva in a background of field-collected copepods. This method could form the basis of a “pond-side test” for detecting potential sources of Guinea worm infection in the environment, in copepods, including in the guts of fish as potential transport hosts, enabling research, surveillance and targeting of control measures. The key constraint on the utility of this assay as a field diagnostic, is a lack of knowledge of variation in the temporal and spatial distribution of D. medinensis larvae in copepods in water bodies in the affected areas and how best to sample copepods to obtain a reliable diagnostic sample. These fundamental knowledge gaps could readily be addressed with field collections of samples across areas experiencing a range of worm infection frequencies, coupled with field and laboratory analyses using LAMP and PCR.

Publication metadata

Author(s): Boonham N, Tomlinson J, Ostoja-Starzewska S, McDonald RA

Publication type: Article

Publication status: Published

Journal: Experimental Parasitology

Year: 2020

Volume: 217

Print publication date: 01/10/2020

Online publication date: 02/08/2020

Acceptance date: 21/07/2020

Date deposited: 15/10/2020

ISSN (print): 0014-4894

ISSN (electronic): 1090-2449

Publisher: Academic Press Inc.


DOI: 10.1016/j.exppara.2020.107960

PubMed id: 32755552


Altmetrics provided by Altmetric


Funder referenceFunder name