Browse by author
Lookup NU author(s): Dr Jim StewartORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
The regulation of mitochondrial RNA processing and its importance for ribosome biogenesis and energy metabolism are not clear. We generated conditional knockout mice of the endoribonuclease component of the RNase P complex, MRPP3, and report that it is essential for life and that heart and skeletal-muscle-specific knockout leads to severe cardiomyopathy, indicating that its activity is non-redundant. Transcriptome-wide parallel analyses of RNA ends (PARE) and RNA-seq enabled us to identify that in vivo 5' tRNA cleavage precedes 3' tRNA processing, and this is required for the correct biogenesis of the mitochondrial ribosomal subunits. We identify that mitoribosomal biogenesis proceeds co-transcriptionally because large mitoribosomal proteins can form a subcomplex on an unprocessed RNA containing the 16S rRNA. Taken together, our data show that RNA processing links transcription to translation via assembly of the mitoribosome.
Author(s): Rackham O, Busch JD, Matic S, Siira SJ, Kuznetsova I, Atanassov I, Ermer JA, Shearwood AMJ, Richman TR, Stewart JB, Mourier A, Milenkovic D, Larsson NG, Filipovska A
Publication type: Article
Publication status: Published
Journal: Cell Reports
Print publication date: 16/08/2016
Online publication date: 04/08/2016
Acceptance date: 13/07/2016
Date deposited: 12/11/2020
ISSN (print): 2211-1247
Publisher: Cell Press
PubMed id: 27498866
Altmetrics provided by Altmetric