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Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines

Lookup NU author(s): Dr Cole SimsORCiD, Dr Adriana BuskinORCiD, Professor Rakesh Heer

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Abstract

© 2021 The AuthorsTwo cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.

Publication metadata

Author(s): Sims CHC, Autio MI, Buskin A, Cheung C, Heer R, Foo RSY, Wang X

Publication type: Article

Publication status: Published

Journal: Stem Cell Research

Year: 2021

Volume: 52

Online publication date: 11/02/2021

Acceptance date: 05/02/2021

Date deposited: 26/03/2021

ISSN (print): 1873-5061

ISSN (electronic): 1876-7753

Publisher: Elsevier B.V.

URL: https://doi.org/10.1016/j.scr.2021.102238

DOI: 10.1016/j.scr.2021.102238

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