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Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota

Lookup NU author(s): Dr Arnaud Basle, Dr Alan Cartmell


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© 2022, The Author(s), under exclusive licence to Springer Nature America, Inc. Sulfated glycans are ubiquitous nutrient sources for microbial communities that have coevolved with eukaryotic hosts. Bacteria metabolize sulfated glycans by deploying carbohydrate sulfatases that remove sulfate esters. Despite the biological importance of sulfatases, the mechanisms underlying their ability to recognize their glycan substrate remain poorly understood. Here, we use structural biology to determine how sulfatases from the human gut microbiota recognize sulfated glycans. We reveal seven new carbohydrate sulfatase structures spanning four S1 sulfatase subfamilies. Structures of S1_16 and S1_46 represent novel structures of these subfamilies. Structures of S1_11 and S1_15 demonstrate how non-conserved regions of the protein drive specificity toward related but distinct glycan targets. Collectively, these data reveal that carbohydrate sulfatases are highly selective for the glycan component of their substrate. These data provide new approaches for probing sulfated glycan metabolism while revealing the roles carbohydrate sulfatases play in host glycan catabolism. [Figure not available: see fulltext.]

Publication metadata

Author(s): Luis AS, Basle A, Byrne DP, Wright GSA, London JA, Jin C, Karlsson NG, Hansson GC, Eyers PA, Czjzek M, Barbeyron T, Yates EA, Martens EC, Cartmell A

Publication type: Article

Publication status: Published

Journal: Nature Chemical Biology

Year: 2022

Volume: 18

Pages: 841-849

Online publication date: 16/06/2022

Acceptance date: 14/04/2022

ISSN (print): 1552-4450

ISSN (electronic): 1552-4469

Publisher: Springer Nature


DOI: 10.1038/s41589-022-01039-x

PubMed id: 35710619


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