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Lookup NU author(s): Yulia Kehayova, Dr Sarah RiceORCiD, Professor John LoughlinORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Objective. Over 100 DNA variants have been associated with osteoarthritis (OA), including rs1046934, located within a linkage disequilibrium block encompassing part of COLGALT2 and TSEN15. Here, we used human foetal cartilage, cartilage from arthroplasty patients, and a chondrocyte cell model to determine the target gene(s) at the locus and the mechanism of action. Methods. Genotyping and methylation array data of cartilage DNA (n=87) were used to determine if rs1046934 genotype associated with differential DNA methylation at proximal CpGs. Results were replicated in arthroplasty (n=132) and foetal (n=77) cartilage DNA using pyrosequencing. Allelic expression imbalance (AEI) measured effect of genotype upon COLGALT2 and TSEN15 expression. Reporter gene assays and epigenetic editing determined the functional role of regions harbouring differentially methylated CpGs. In silico analyses complemented these experiments. Results. Three differentially methylated CpGs residing within regulatory regions were detected, two of which replicated. AEI was detected for COLGALT2 and TSEN15, with associations between expression and methylation for COLGALT2. Reporter assays confirmed that the CpGs are in chondrocyte enhancers with epigenetic editing directly linking methylation with COLGALT2 expression. Conclusion. COLGALT2 is a target of this OA locus. We previously characterised another OA locus, marked by rs11583641, that independently targets COLGALT2. rs1046934, like rs11583641, mediates its effect by modulating expression of COLGALT2 via methylation changes to CpGs located in enhancers. The SNPs, CpGs and enhancers are distinct between the loci but the effect on COLGALT2 is the same. COLGALT2 is the target of independent OA risk loci sharing a common mechanism of action.
Author(s): Kehayova YS, Wilkinson JM, Rice SJ, Loughlin J
Publication type: Article
Publication status: Published
Journal: Arthritis & Rheumatology
Year: 2023
Volume: 75
Issue: 6
Pages: 910-922
Print publication date: 01/06/2023
Online publication date: 20/12/2022
Acceptance date: 15/12/2022
Date deposited: 22/12/2022
ISSN (print): 2326-5191
ISSN (electronic): 2326-5205
Publisher: John Wiley & Sons, Inc.
URL: https://doi.org/10.1002/art.42427
DOI: 10.1002/art.42427
PubMed id: 36538011
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