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Identification of a protein expression signature distinguishing early from organising diffuse alveolar damage in COVID-19 patients

Lookup NU author(s): Dr Luke Milross, Dr Joaquim Majo, Bethany Hunter, Professor Andrew FilbyORCiD, Professor Andrew FisherORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY. Published by BMJ.Diffuse alveolar damage (DAD) is the histological expression of acute respiratory distress syndrome and characterises lung pathology due to infection with SARS-CoV-2, and other respiratory pathogens of clinical significance. DAD reflects a time-dependent immunopathological process, progressing from an early/exudative stage through to an organising/fibrotic stage, yet within an individual these different stages of DAD may coexist. Understanding the progression of DAD is central to the development of new therapeutics to limit progressive lung damage. Here, we applied highly multiplexed spatial protein profiling to autopsy lung tissues derived from 27 patients who died from COVID-19 and identified a protein signature (ARG1, CD127, GZMB, IDO1, Ki67, phospho-PRAS40 (T246) and VISTA) that distinguishes early DAD from late DAD with good predictive accuracy. These proteins warrant further investigation as potential regulators of DAD progression.

Publication metadata

Author(s): Ashwin H, Milross L, Wilson J, Majo J, Hang Lee JT, Calder G, Hunter B, James S, Lagos D, Signoret N, Filby A, Bayraktar OA, Fisher AJ, Kaye PM

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Pathology

Year: 2023

Volume: 76

Issue: 8

Pages: 561-565

Online publication date: 09/03/2023

Acceptance date: 09/02/2023

Date deposited: 03/05/2023

ISSN (print): 0021-9746

ISSN (electronic): 1472-4146

Publisher: BMJ Publishing Group


DOI: 10.1136/jcp-2023-208771

PubMed id: 36894313


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