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Lookup NU author(s): Parmveer Singh, Hannah Kendall, Laura WilsonORCiD, Ryan Long, Dr Adriana BuskinORCiD, Dr Colin Miles, Professor Rakesh Heer, Professor Craig Robson
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© 2023. Published by The Company of Biologists Ltd. The reactivation of developmental genes and pathways during adulthood may contribute to pathogenesis of diseases such as prostate cancer. Analysis of the mechanistic links between development and disease could be exploited to identify signalling pathways leading to disease in the prostate. However, the mechanisms underpinning prostate development require further characterisation to interrogate fully the link between development and disease. Previously, our group developed methods to produce prostate organoids using induced pluripotent stem cells (iPSCs). Here, we show that human iPSCs can be differentiated into prostate organoids using neonatal rat seminal vesicle mesenchyme in vitro. The organoids can be used to study prostate development or modified to study prostate cancer. We also elucidated molecular drivers of prostate induction through RNA-sequencing analyses of the rat urogenital sinus and neonatal seminal vesicles. We identified candidate drivers of prostate development evident in the inductive mesenchyme and epithelium involved with prostate specification. Our top candidates included Spx, Trib3, Snai1, Snai2, Nrg2 and Lrp4. This work lays the foundations for further interrogation of the reactivation of developmental genes in adulthood, leading to prostate disease.
Author(s): Singh P, Lanman NA, Kendall HLR, Wilson L, Long R, Franco OE, Buskin A, Miles CG, Hayward SW, Heer R, Robson CN
Publication type: Article
Publication status: Published
Journal: Development
Year: 2023
Volume: 150
Issue: 13
Online publication date: 12/07/2023
Acceptance date: 20/06/2023
ISSN (print): 0950-1991
ISSN (electronic): 1477-9129
Publisher: The Company of Biologists Ltd
URL: https://doi.org/10.1242/dev.201328
DOI: 10.1242/dev.201328
PubMed id: 37376888
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