ePrints

Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light

Lookup NU author(s): Professor Majlinda LakoORCiD, Professor Evelyne SernagorORCiD

Downloads

• Published version [.pdf]

Licence

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).

Abstract

© 2023 The Author(s)Cells in the human retina must rapidly adapt to constantly changing visual stimuli. This fast adaptation to varying levels and wavelengths of light helps to regulate circadian rhythms and allows for adaptation to high levels of illumination, thereby enabling the rest of the visual system to remain responsive. It has been shown that retinal microRNA (miRNA) molecules play a key role in regulating these processes. However, despite extensive research using various model organisms, light-regulated miRNAs in human retinal cells remain unknown. Here, we aim to characterize these miRNAs. We generated light-responsive human retinal organoids that express miRNA families and clusters typically found in the retina. Using an in-house developed photostimulation device, we identified a subset of light-regulated miRNAs. Importantly, we found that these miRNAs are differentially regulated by distinct wavelengths of light and have a rapid turnover, highlighting the dynamic and adaptive nature of the human retina.

Publication metadata

Author(s): Celiker C, Weissova K, Cerna KA, Oppelt J, Dorgau B, Gambin FM, Sebestikova J, Lako M, Sernagor E, Liskova P, Barta T

Publication type: Article

Publication status: Published

Journal: iScience

Year: 2023

Volume: 26

Issue: 7

Print publication date: 21/07/2023

Online publication date: 28/06/2023

Acceptance date: 25/06/2023

Date deposited: 08/09/2023

ISSN (electronic): 2589-0042

Publisher: Elsevier Inc.

URL: https://doi.org/10.1016/j.isci.2023.107237

DOI: 10.1016/j.isci.2023.107237

Data Access Statement: Data and code availability • Bulk miRNA sequencing data have been deposited at GEO and are publicly available as of the date of publication. Accession numbers are listed in the key resources table. Individual data points from qPCR analysis have been deposited at Mendeley and are publicly available as of the date of publication. The DOI is listed in the key resources table. • This paper does not report original code. • Any additional information required to reanalyze the data deposited in this paper is available from the lead contact upon request.

Altmetrics

Funding

Share