Toggle Main Menu Toggle Search

Open Access padlockePrints

Pre-gelatinized high-amylose starch enables easy preparation of flexible and antimicrobial composite films for fresh fruit preservation

Lookup NU author(s): Dr Peng Liu, Dr David XieORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


While high-amylose starch (HAS) possesses advantageous properties such as high resistant starch content and favorable mechanical attributes, its gelatinization constraints have limited its applicability. This study enhances its versatility by focusing on pre-gelatinized (PG) HAS with exceptional rehydratability, achieved by disorganizing native HAS granules (with amylose contents of 55 % and 68 %, respectively) using a 33 % CaCl2 solution, followed by water-ethanol precipitation and freeze-drying. The resulting PG-HAS exhibited elevated amylose content (61 % and 75 %) with minimal changes in amylose molecular weight. PG-HAS displayed superior water-absorption index (WAI) and water-soluble index (WSI) compared to native HAS, further improved by 2 % CaCl2 solution incorporation. Furthermore, composite films were prepared by mixing PG-HAS with PVA at a 6:4 (w/w) ratio. The PG-G50 (61 % amylose content)/PVA composite film exhibited remarkable elongation (131.1 ± 5.4 %), nearly three times that of a normal corn starch (NCS, with 27 % amylose)/PVA film, attributed to improved starch dispersity and higher amylose content. Nonetheless, the PG-G70 (75 % amylose content)/PVA film at the same ratio showed lower elongation (54.7 ± 8.0 %), potentially due to strong cohesive forces between amylose chains that impede starch-PVA interactions. Moreover, the PG-HAS/PVA composite films, enriched with antibacterial agents, demonstrated effective antibacterial properties with a gradual and sustained release of active compounds. Notably, the PG-G50/PVA/tannic acid (TA) film effectively preserved fresh apple slices by inhibiting bacteria growth and preventing browning. These findings underscore the excellent rehydration of PG-HAS and its potential as an inner packaging material for irregularly shaped foods, such as sliced fruits or meats, due to its nontoxic nature, softness and flexibility, which allows the film to maintain close contact with food surfaces.

Publication metadata

Author(s): Xi W, Liu P, Ling J, Xian D, Wu L, Yuan Y, Zhang J, Xie F

Publication type: Article

Publication status: Published

Journal: International Journal of Biological Macromolecules

Year: 2023

Volume: 254

Issue: Part 2

Print publication date: 01/01/2024

Online publication date: 07/11/2023

Acceptance date: 05/11/2023

Date deposited: 11/11/2023

ISSN (print): 0141-8130

ISSN (electronic): 1879-0003

Publisher: Elsevier


DOI: 10.1016/j.ijbiomac.2023.127938


Altmetrics provided by Altmetric


Funder referenceFunder name
China Scholarship Council (CSC)
Special Fund for Scientific and Technological Innovation in Guangdong Province