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Lookup NU author(s): Moyassar Al-Shaibani, Dr Xiao Wang, Dr Asif Tulah, Dr Rachel CrosslandORCiD, Professor Anne Dickinson, Professor Penny Lovat
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© 2024 MA Healthcare Ltd. All rights reserved. Alternative 3-dimensional (3D) skin models that replicate in vivo human skin are required to investigate important events during wound healing, such as collective cell migration, epidermal layer formation, dermal substrate formation, re-epithelialisation and collagen production. In this study, a matched human 3D skin equivalent model (3D-SEM) was developed from human skin cells (fibroblast and keratinocytes), characterised using haematoxylin and eosin, immunofluorescence staining and microRNA profiling. The 3D-SEM was then functionally tested for its use in wound healing studies. Mesenchymal stem cells (MSCs) were isolated and characterised according to the criteria stipulated by the International Society for Cell Therapy. Cytokine and growth factor secretions were analysed by enzyme-linked immunosorbent assay. MSC-conditioned medium (MSC-CM) was then tested for wound healing capacity using the developed 3D-SEM at different timepoints i.e., at one, two and four weeks. The constructed 3D-SEM showed consistent development of skin-like structures composed of dermal layers and epidermal layers, with the ability to express epidermal differentiation markers and full stratification. They also showed prolonged longevity in culture media, retaining full differentiation and stratification within the four weeks. MicroRNA profiling revealed a strong correlation in microRNA expression between the developed 3D-SEM and the original native skin (p<0.001; R=0.64). Additionally, MSC-CM significantly enhanced migration, proliferation and differentiation of epidermal cells in the wounded models compared to control models at the different timepoints. In conclusion, in this study, the developed 3D-SEM mimicked native skin at the cellular and molecular levels, and clearly showed the important stages of skin regeneration during the healing process. MSC secretome contains growth factors that play a pivotal role in the healing process and could be used as a therapeutic option to accelerate skin healing. Declaration of interest: The authors have no conflicts of interest.
Author(s): Al-Shaibani M, Wang X-N, Tulah A, Crossland RE, Dickinson AM, Lovat PE
Publication type: Article
Publication status: Published
Journal: Journal of Wound Care
Year: 2024
Volume: 33
Pages: clxxxii-cxciii
Online publication date: 20/08/2024
Acceptance date: 02/04/2018
ISSN (print): 0969-0700
ISSN (electronic): 2052-2916
Publisher: MA Healthcare Ltd
URL: https://doi.org/10.12968/jowc.2021.0397
DOI: 10.12968/jowc.2021.0397
PubMed id: 39163155
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