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Lookup NU author(s): Professor Wyatt YueORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Copyright © 2022 Coker, Katis, Fairhead, Schwenzer, Clemmensen, Frandsen, de Jongh, Gileadi, Burgess-Brown, Marsden, Midwood and Yue. Recombinant protein expression in eukaryotic insect cells is a powerful approach for producing challenging targets. However, due to incompatibility with standard baculoviral platforms and existing low-throughput methodology, the use of the Drosophila melanogaster “S2” cell line lags behind more common insect cell lines such as Sf9 or High-Five™. Due to the advantages of S2 cells, particularly for secreted and secretable proteins, the lack of a simple and parallelizable S2-based platform represents a bottleneck, particularly for biochemical and biophysical laboratories. Therefore, we developed FAS2FURIOUS, a simple and rapid S2 expression pipeline built upon an existing low-throughput commercial platform. FAS2FURIOUS is comparable in effort to simple E. coli systems and allows users to clone and test up to 46 constructs in just 2 weeks. Given the ability of S2 cells to express challenging targets, including receptor ectodomains, secreted glycoproteins, and viral antigens, FAS2FURIOUS represents an attractive orthogonal approach for protein expression in eukaryotic cells.
Author(s): Coker JA, Katis VL, Fairhead M, Schwenzer A, Clemmensen SB, Frandsen BU, de Jongh WA, Gileadi O, Burgess-Brown NA, Marsden BD, Midwood KS, Yue WW
Publication type: Article
Publication status: Published
Journal: Frontiers in Bioengineering and Biotechnology
Year: 2022
Volume: 10
Online publication date: 05/05/2022
Acceptance date: 04/04/2022
Date deposited: 12/09/2024
ISSN (electronic): 2296-4185
Publisher: Frontiers Media S.A.
URL: https://doi.org/10.3389/fbioe.2022.871933
DOI: 10.3389/fbioe.2022.871933
Data Access Statement: The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article.
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