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Lookup NU author(s): Professor Tiago OuteiroORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2024 The AuthorsAggregation of alpha-synuclein (aSyn) occurs in presynaptic neurons and constitutes a key factor for the progression of Parkinson's disease, emphasising the urgency of early detection to support effective treatment. Unfortunately, a reliable, sensitive and cost-effective diagnostic tool has so far been lacking. Thus, this work presents a novel biosensor for detecting aSyn using plastic antibodies coupled to electrochemical detection. This biosensor was designed for portability and compatibility with point-of-care devices and exploits the electropolymerization of methylene blue (MB) together with aSyn on the carbon working electrode of screen-printed electrodes (SPEs). By electrochemical impedance spectroscopy (EIS) measurements, the sensor showed exceptional analytical performance in detecting aSyn monomers in human CSF samples. It showed a linear trend of response from 1 fM to 10 pM with an impressively low limit of detection of 69 aM. Selectivity tests confirmed the predominant response to aSyn monomers, a less intense response to oligomers and insensitivity to fibrils. Overall, this plastic antibody-based electrochemical sensor represents a significant breakthrough as it is the first of its kind to accurately, sensitively and selectively detect aSyn monomers with a partial response to oligomers. Its simplicity and reproducibility promise to contribute to the early and effective diagnosis of Parkinson's disease.
Author(s): da Silva IS, Cardoso AR, Reimer L, Konig A, van Riesen C, Outeiro TF, Jensen PH, Sales MGF
Publication type: Article
Publication status: Published
Journal: Biosensors and Bioelectronics
Year: 2025
Volume: 268
Print publication date: 15/01/2025
Online publication date: 06/11/2024
Acceptance date: 24/10/2024
Date deposited: 19/11/2024
ISSN (print): 0956-5663
ISSN (electronic): 1873-4235
Publisher: Elsevier Ltd
URL: https://doi.org/10.1016/j.bios.2024.116880
DOI: 10.1016/j.bios.2024.116880
Data Access Statement: No data was used for the research described in the article.
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