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Lookup NU author(s): Professor Tiago OuteiroORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2024 Shared Science Publishers OG. All rights reserved.Stress granules (SGs) are highly dynamic micromolecular membraneless condensates that generate in cells subjected to stress. Formed from pools of untranslating messenger ribonucleoproteins (RNP), SGs dynamics constitute vital processes essential for cell survival. Here, we investigate whether established cytotoxic agents, such as the platinum-based chemotherapeutic agent cisplatin and the aminoglycoside antibiotic gentamicin, elicit SG formation in the House Ear Institute-Organ of Corti-1 (HEI-OC1) auditory cell line, H4 human neuroglioma cells and HEK-293T human embryonic kidney cells. Cells were treated with cisplatin or gentamicin for specific durations at designated concentrations. SG formation was assessed using immunocytochemistry and live cell imaging. Levels of essential proteins involved in SG assembly were evaluated using immunoblotting. We observed cisplatin-associated SG assembly in HEI-OC1 and H4 cells via confocal microscopy through antibody colabeling of G3BP1 with PABP or Caprin1. While maintaining an unchanged pattern of expression of main constituent SG proteins, cisplatin-related SGs in H4 cells persisted for at least 12 h after drug removal. Cells subjected to gentamicin exposure did not exhibit SGs. Our findings offer insights into subcellular mechanisms related to cisplatin-associated cytotoxicity, highlighting the need for future studies to further investigate this stress-response mechanism.
Author(s): Abdelrasol H, Chopra A, Shvachiy L, Beutner D, Outeiro TF, Setz C
Publication type: Article
Publication status: Published
Journal: Cell Stress
Year: 2024
Volume: 8
Pages: 83-98
Online publication date: 18/10/2024
Acceptance date: 04/09/2024
Date deposited: 25/11/2024
ISSN (electronic): 2523-0204
Publisher: Shared Science Publishers OG
URL: https://doi.org/10.15698/cst2024.10.299
DOI: 10.15698/cst2024.10.299
Data Access Statement: The authors state that all data necessary for confirming the conclusions presented in the manuscript are represented fully within the manuscript
