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Lookup NU author(s): Dr Soren NielsenORCiD, Professor Nikolay ZenkinORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2026 Kuś et al. Published by Cold Spring Harbor Laboratory Press for the RNA Society. Maturation of protein-coding precursor messenger RNA (pre-mRNA) is closely linked to RNA polymerase II (Pol II) transcription. However, the mechanistic understanding of how pre-mRNA processing is coordinated with transcription remains incomplete. Conserved proteins interacting with the C-terminal domain of the largest catalytic subunit of Pol II and nascent RNA (CID-RRM factors) were demonstrated to play a role in pre-mRNA 3'-end processing and termination of Pol II transcription. Here, we use a fully reconstituted system to demonstrate that the fission yeast CID-RRM factor Seb1 acts as a bona fide elongation factor. Our analyses show that Seb1 exhibits context-dependent regulation of Pol II pausing, capable of either promoting or inhibiting pause site entry. We propose that CID-RRM factors coordinate Pol II transcription and pre-mRNA 3'-end processing by modulating the rate of Pol II transcription.
Author(s): Kus K, Nielsen S, Zenkin N, Vasiljeva L
Publication type: Article
Publication status: Published
Journal: RNA
Year: 2025
Volume: 32
Pages: 71-81
Online publication date: 24/10/2025
Acceptance date: 04/10/2025
Date deposited: 05/01/2026
ISSN (print): 1355-8382
ISSN (electronic): 1469-9001
Publisher: Cold Spring Harbor Laboratory Press
URL: https://doi.org/10.1261/rna.080765.125
DOI: 10.1261/rna.080765.125
Data Access Statement: Supplemental material is available for this article at https://rnajournal.cshlp.org/content/32/1/71/suppl/DC1
PubMed id: 41136340
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