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Lookup NU author(s): Dr Eilidh Mackenzie, Dr Kacper Sendra, Dr Arnaud Basle, Dr Kevin Waldron
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© The Author(s) 2026. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution. Changes in protein properties and functions are central to the evolution of life. Metalloproteins can evolve by changing their preference from one metal cofactor to another. Recently, we demonstrated that the widely distributed iron- or manganese-dependent superoxide dismutase (SodFM) family has undergone numerous metal-preference changes, including during evolutionary adaptation of pathogenic bacteria to altered metal availability within the host. Yet the underlying properties of metal-binding sites that control metalloenzyme metal preference are unclear, and thus, we lack an understanding of how enzymatic metal preference can be reshaped by evolution. Here, we used spectral features of bound iron or manganese, whose intensities reflect their oxidation state, to assess how their redox properties are tuned during SodFM evolution. We systematically analyzed the metal oxidation state across diverse SodFMs from multiple phylogenetic groups with different catalytic metal preferences, including those known to have undergone evolutionary metal-preference switching. We observed a striking relationship between resting oxidation state and catalytic metal preferences. Mutagenesis of second-sphere residues previously identified as determining metal preference revealed that they modulate metal-dependent activity and cofactor oxidation state in tandem, demonstrating these properties are linked. Together, these data argue that the differing SodFM metal preferences observed across the tree of life evolved through tuning of their redox properties by the secondary coordination sphere. This study gives insight into the process by which a metalloenzyme originally optimized for one metal cofactor can evolve a new metal preference, under suitable selection pressure, through re-optimization of its active site for catalytic reactivity of the new metal cofactor.
Author(s): Mackenzie ES, Sendra KM, Basle A, Mazgaj R, Kehl-Fie TE, Waldron KJ
Publication type: Article
Publication status: Published
Journal: Molecular Biology and Evolution
Year: 2026
Volume: 43
Issue: 3
Print publication date: 01/03/2026
Online publication date: 13/02/2026
Acceptance date: 04/02/2026
Date deposited: 13/04/2026
ISSN (electronic): 1537-1719
Publisher: Oxford University Press
URL: https://doi.org/10.1093/molbev/msag040
DOI: 10.1093/molbev/msag040
Data Access Statement: Source data underpinning this study is included in the Source Data file, available online. All additional raw data will be available on request. Computational analyses used publicly available data derived from NCBI. The crystallography data is available from the Protein Databank (PDB).
PubMed id: 41684149
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