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The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family: a potential role in the regulation of higher-order chromatin structure

Lookup NU author(s): Dr Ian Cowell

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Abstract

The expression of the nuclear protein Ki-67 (pKi-67) is strictly correlated with cell proliferation. Because of this, anti-Ki-67 antibodies can be used as operational markers to estimate the growth fraction of human neoplasia in situ. For a variety of tumours, the assessment of pKi-67 expression has repeatedly been proven to be of prognostic value for survival and tumour recurrence, but no cellular function has yet been ascribed to the Ki-67 protein. This study shows that a C-terminal domain of pKi-67 (Kon21) is able to bind to all three members of the mammalian heterochromatin protein 1 (HP1) family in vitro and in vivo. This interaction can be manipulated in living cells, as evidenced by ectopic expression of GFP-tagged HP1 proteins in HeLa cells, which results in a dramatic relocalization of endogenous pKi-67. Taken together, the data presented in this study suggest a role for pKi- 67 in the control of higher-order chromatin structure


Publication metadata

Author(s): Scholzen T, Endl E, Wohlenberg C, Sar SvanDer, Cowell IG, Gerdes J, Singh PB

Publication type: Article

Publication status: Published

Journal: Journal of Pathology

Year: 2002

Volume: 196

Issue: 2

Pages: 135-144

ISSN (print): 0022-3417

ISSN (electronic): 1096-9896

Publisher: John Wiley & Sons Ltd.

URL: http://dx.doi.org/10.1002/path.1016

DOI: 10.1002/path.1016

PubMed id: 11793364


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