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Lookup NU author(s): Dr Janet Lindsey
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Lambda red gam phage carrying a 571 base-pair palindrome are unviable in wild-type Escherichia coli hosts. By using de-methylation to study the fate of DNA strands introduced into E. coli, we have demonstrated that a decrease in the yield of palindrome-containing molecules with two newly synthesized strands can occur without any concomitant loss of replicated molecules containing input strands. This implies that the palindrome-containing DNA is not being destroyed even as a consequence of replication, but rather that its replication rate is reduced. These results demonstrate that a palindrome can mediate unviability without directing cleavage of its carrier replicon.
Author(s): Lindsey JC, Leach DRF
Publication type: Letter
Publication status: Published
Journal: Journal of Molecular Biology
Print publication date: 20/04/1989
ISSN (print): 0022-2836
ISSN (electronic): 1089-8638
PubMed id: 2738