Toggle Main Menu Toggle Search

Open Access padlockePrints

Slow replication of palindrome-containing DNA

Lookup NU author(s): Dr Janet Lindsey


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Lambda red gam phage carrying a 571 base-pair palindrome are unviable in wild-type Escherichia coli hosts. By using de-methylation to study the fate of DNA strands introduced into E. coli, we have demonstrated that a decrease in the yield of palindrome-containing molecules with two newly synthesized strands can occur without any concomitant loss of replicated molecules containing input strands. This implies that the palindrome-containing DNA is not being destroyed even as a consequence of replication, but rather that its replication rate is reduced. These results demonstrate that a palindrome can mediate unviability without directing cleavage of its carrier replicon.

Publication metadata

Author(s): Lindsey JC, Leach DRF

Publication type: Letter

Publication status: Published

Journal: Journal of Molecular Biology

Year: 1989

Volume: 206

Issue: 4

Pages: 779-782

Print publication date: 20/04/1989

ISSN (print): 0022-2836

ISSN (electronic): 1089-8638


DOI: 10.1016/0022-2836(89)90584-6

PubMed id: 2738