Toggle Main Menu Toggle Search

Open Access padlockePrints

In vivo loss of supercoiled DNA carrying a palindromic sequence

Lookup NU author(s): Dr Janet Lindsey


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Interest in the fate of long palindromic DNA sequences in E. coli has been kindled by the observation that their inviability is overcome in recBC sbcB strains and that these hosts permit the construction of DNA libraries containing long palindromic sequences present in the human genome. In this paper we show that a reduction in the level of intracellular supercoiled DNA occurs as the result of the presence of a 530 bp palindrome in bacteriophage lambda. This reduction occurs in Rec+ and recA strains but not in strains lacking exonucleases V and I (recBC sbcB). However, the DNA must be active (not repressed) for this reduction to be observed, since it is not seen in a Rec+ host lysogenic for phage lambda. These results argue against two hypotheses: firstly, that the palindrome causes inviability solely by interfering with packaging, and secondly, that it does so solely by interfering with recombination. Conversely, these results suggest that a feature of active monomeric DNA (probably its replication) is involved in inviability.

Publication metadata

Author(s): Leach DRF, Lindsey JC

Publication type: Article

Publication status: Published

Journal: Molecular and General Genetics

Year: 1986

Volume: 204

Issue: 2

Pages: 322-327

Print publication date: 01/08/1986

ISSN (print): 0026-8925

ISSN (electronic): 1432-1874

Publisher: Springer Berlin


DOI: 10.1007/BF00425517

PubMed id: PMID: 2945


Altmetrics provided by Altmetric