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Detection of poly(ADP-ribose) synthesis in Drosophila testes upon gamma-irradiation

Lookup NU author(s): Professor Alexander Burkle


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Poly(ADP-ribose) polymerase (PARP) activity is widespread among eukaryotes. Upon DNA damage PARP binds to DNA strand breaks and transfers ADP-ribose residues from NAD+ to acceptor proteins and to ADP-ribosyl protein adducts. This leads to branched polymers of protein-coupled poly(ADP-ribose) (pADPr). Because the germline of Drosophila has recently become important in the study of DNA double-strand break repair (DSBR) as opposed to somatic DSBR we tested whether the catalytic activity of PARP can be stimulated by g-irradiation during Drosophila spermatogenesis. Using antibodies against pADPr we detected a significant increase in PARP activity in male germline cells during spermatogenesis upon g-irradiation. Different stages of spermatogenesis revealed different subnuclear localization patterns of pADPr. In premeiotic and postmeiotic cells pADPr localized in a pattern overlapping with lamin and topoisomerase II at the nuclear rim. In primary spermatocytes pADPr is associated with three loci corresponding to thechromosomes at the nuclear periphery.

Publication metadata

Author(s): Lankenau S, Bürkle A, Lankenau DH

Publication type: Article

Publication status: Published

Journal: Chromosoma

Year: 1999

Volume: 108

Issue: 1

Pages: 44-51

Print publication date: 01/01/1999

ISSN (print): 0009-5915

ISSN (electronic): 1432-0886

Publisher: Springer


DOI: 10.1007/s004120050350


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