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Lookup NU author(s): Professor Alexander Burkle
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Poly(ADP-ribose) polymerase (PARP) activity is widespread among eukaryotes. Upon DNA damage PARP binds to DNA strand breaks and transfers ADP-ribose residues from NAD+ to acceptor proteins and to ADP-ribosyl protein adducts. This leads to branched polymers of protein-coupled poly(ADP-ribose) (pADPr). Because the germline of Drosophila has recently become important in the study of DNA double-strand break repair (DSBR) as opposed to somatic DSBR we tested whether the catalytic activity of PARP can be stimulated by g-irradiation during Drosophila spermatogenesis. Using antibodies against pADPr we detected a significant increase in PARP activity in male germline cells during spermatogenesis upon g-irradiation. Different stages of spermatogenesis revealed different subnuclear localization patterns of pADPr. In premeiotic and postmeiotic cells pADPr localized in a pattern overlapping with lamin and topoisomerase II at the nuclear rim. In primary spermatocytes pADPr is associated with three loci corresponding to thechromosomes at the nuclear periphery.
Author(s): Lankenau S, Bürkle A, Lankenau DH
Publication type: Article
Publication status: Published
Journal: Chromosoma
Year: 1999
Volume: 108
Issue: 1
Pages: 44-51
Print publication date: 01/01/1999
ISSN (print): 0009-5915
ISSN (electronic): 1432-0886
Publisher: Springer
URL: http://dx.doi.org/10.1007/s004120050350
DOI: 10.1007/s004120050350
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