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New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry

Lookup NU author(s): Professor Debra Bevitt, Emeritus Professor Geoffrey Toms, Professor Thomas Lennard, Professor Bruce Westley, Dr Brian Angus


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Assessment of oestrogen and progesterone receptors (ER and PgR) in breast cancer is widely used for the prediction of response to endocrine therapy and as a prognostic marker. Cytosolic assays have been replaced in many centres by immunochemical techniques, which have many advantages including applicability to small samples, simplicity, and cost-effectiveness. This study describes the generation and characterisation of two novel murine monoclonal antibodies recognizing ER and PgR, designated NCL-ER-6F11 and NCL- PGR respectively, which are effective in heat-treated formalin-fixed, paraffin-embedded tissue. The antibodies have been characterized by Western blotting and by immunohistochemistry on normal and pathological breast and other tissues. NCL-ER-6F11 has been shown to compare favourably with a currently available ER antibody. These antibodies may prove of value in the assessment of hormone receptor status in human breast cancer.

Publication metadata

Author(s): Bevitt D, Milton I, Piggot N, Henry L, Carter M, Toms G, Lennard TWJ, Westley B, Angus B, Horne C

Publication type: Article

Publication status: Published

Journal: Journal of Pathology

Year: 1997

Volume: 183

Issue: 2

Pages: 228-232

Print publication date: 01/10/1997

ISSN (print): 0022-3417

ISSN (electronic): 1096-9896


DOI: 10.1002/(SICI)1096-9896(199710)183:2<228::AID-PATH895>3.0.CO;2-X

PubMed id: 9390038


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