Toggle Main Menu Toggle Search

Open Access padlockePrints

Uptake and processing of glycosylated mycolates for presentation to CD1b-restricted T cells

Lookup NU author(s): Professor Del Besra


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Antigen presenting cells (APCs) expressing CD1b mediate the specific T cell recognition of mycobacterial lipid antigens. These lipid antigens require internalization by APCs prior to presentation, but the detailed mechanisms of uptake and intracellular processing are not known. Here we have examined several steps in the presentation of two related classes of CD1b- presented antigens, free and glycosylated mycolates. T cell recognition of glucose monomycolate (GMM) was blocked by agents that fix APC membranes or neutralize the pH of endosomes, indicating a requirement for GMM uptake into an acidic compartment prior to recognition. Different T cell lines responded to free mycolate or GMM without crossreactivity, yet both antigens were taken up by APCs at the same rate. This demonstrated that differential recognition of these antigens resulted from T cell specificity for their hydrophilic caps and that APCs were unable to interconvert these antigens by enzymatic or chemical deglycosylation or glycosylation. APCs were also unable to cleave mycobacterial trehalose dimycolate (TDM) at its most chemically labile linkages to yield antigenic free mycolates or GMM. Our results indicate that these mycolate-containing antigens are resistant to chemical or enzymatic cleavage by APCs, suggesting that molecular trimming is not a universal feature of lipid antigen processing.

Publication metadata

Author(s): Moody DB, Reinhold BB, Reinhold VN, Besra GS, Porcelli SA

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Immunology Letters

Year of Conference: 1999

Pages: 85-91

ISSN: 0165-2478

Publisher: Elsevier


DOI: 10.1016/S0165-2478(98)00129-1

PubMed id: 10065632

Library holdings: Search Newcastle University Library for this item

ISBN: 18790542