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Bacterial xylanase expression in mammalian cells and transgenic mice

Lookup NU author(s): Professor Simi Ali, Emeritus Professor Harry Gilbert, Emeritus Professor Barry Hirst, Dr Judith Hall

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Abstract

The energy which simple-stomached livestock can derive from dietary plant material is limited by the lack of plant polysaccharide degrading enzymes in their gastro-intestinal (GI) tract and the inefficient microbial fermentation of such material in their hind-gut. In poultry the non-starch polysaccharides found in cereal grains can also impair normal digestive function as they form viscous gels in the GI tract inhibiting the breakdown and absorption of nutrients. The nutrition of such livestock could, therefore, be improved by the introduction of enzymes able to degrade plant polysaccharides in the small intestine. We describe the expression of a xylanase, XYLY', from the bacterium Clostridium thermocellum in mammalian cells and the exocrine pancreas of transgenic mice. The enzyme is synthesised, secreted and functionally active in the eukaryote system. This work demonstrates the feasibility of generating animals with the endogenous capacity to depolymerise the xylan component of hemi-cellulose. Copyright (C) 1999 Elsevier Science B.V.


Publication metadata

Author(s): Ali S; Hirst BH; Hall J; Gilbert HJ; Fontes CMGA; Hazlewood GP

Publication type: Article

Publication status: Published

Journal: Journal of Biotechnology

Year: 1999

Volume: 72

Issue: 1-2

Pages: 95-101

Print publication date: 11/06/1999

ISSN (print): 0168-1656

ISSN (electronic):

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/S0168-1656(99)00098-X

DOI: 10.1016/S0168-1656(99)00098-X

PubMed id: 10406100


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