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Crystallization and preliminary X-ray analysis of arabinanase A from Pseudomonas fluorescens subspecies cellulosa

Lookup NU author(s): Emeritus Professor Harry Gilbert

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Abstract

Crystals of 1,5-α-arabinanase A from Pseudomonas fluorescens subspecies cellulosa have been obtained by vapour diffusion. The crystals belong to the space group P6122 with unit-cell parameters a = b = 91.6, c = 179.4 Å with one molecule in the asymmetric unit. The native crystals and, to a much greater extent, heavy-atom soaked crystals are sensitive to radiation which necessitates cryocooling. Suitable cryocooling conditions have been established, though a shrinkage of the unit cell is observed, with a = b = 88.8 and c = 176.9 Å.

Publication metadata

Author(s): Scott M, Pickersgill RW, Hazlewood GP, Gilbert HJ, Harris GW

Publication type: Article

Publication status: Published

Journal: Acta Crystallographica Section D: Biological Crystallography

Year: 1999

Volume: 55

Issue: 2

Pages: 544-546

Print publication date: 01/02/1999

ISSN (print): 0907-4449

ISSN (electronic): 1399-0047

Publisher: Wiley-Blackwell Publishing, Inc.

URL: http://dx.doi.org/10.1107/S0907444998011500

DOI: 10.1107/S0907444998011500

PubMed id: 10089373

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