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Lookup NU author(s): Dr Helen Robertson,
Emeritus Professor John Kirby,
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Chronic rejection is a major cause of graft dysfunction following kidney transplantation. This fibroproliferative disease may be promoted by overproduction of transforming growth factor beta (TGF-β). Previous studies have suggested that cyclosporin-A (CyA) might increase production of this growth factor. The current study was designed to measure the expression of TGF-β in renal transplant biopsies from patients immunosuppressed with either CyA or tacrolimus. Paraffin-embedded renal biopsies were sectioned, dewaxed and incubated with primary antibody against active TGF-β1 antibody. After washing, the sections were treated with secondary antibody conjugated with fluorescein isothiocyanate (FITC). In each case the sections were assessed by semi-quantitative scanning laser confocal microscopy. Biopsies from patients receiving CyA expressed significantly more active TGF-β1 than biopsies from patients receiving tacrolimus (P < 0.0001, Mann-Whitney test). The increased level of active TGF-β1 expression in renal biopsies of patients receiving CyA may indicate a mechanism of chronic rejection. © Springer-Verlag 2000.
Author(s): Mohamed MAS, Robertson H, Booth TA, Balupuri S, Gerstenkorn C, Kirby JA, Talbot D
Publication type: Article
Publication status: Published
Journal: Transplant International
ISSN (print): 0934-0874
ISSN (electronic): 1432-2277
Publisher: John Wiley & Sons
PubMed id: 11112017
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