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Apoptosis in neuroblastomas induced by interferon-γ involves the CD95/CD95L pathway

Lookup NU author(s): Professor Penny Lovat, Dr Chris RedfernORCiD, Professor Andrew Pearson


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Background and Procedure. To determine the possible role of Fas/FasL system in the particularly heterogeneous behaviour of neuroblastoma (NB), we have measured the functional expression of Fas and its ligand, FasL, in primary neuroblastoma samples and cell lines by immunohistochemistry and flow cytometry. Results. Our results reveal that while Fas expression is associated with low stage and more mature tumors, heterogeneous FasL expression was mostly detected in high stage tumors, without apparent correlation to MYCN amplification. Flow cytometric analysis of cell lines demonstrated a high expression of Fas in epithelial-type, HLA class I positive cell lines, which was lost upon activation with phorbol esters. In contrast, Fas ligand was detected in only a small subset of cell lines. Conclusions. In some cell lines, cytotoxic assays revealed the ability of NB-associated Fas receptor to transduce an apoptotic signal upon triggering. The pattern of functional Fas/FasL expression in tumours and cell lines suggests that this system may be involved in the evasion of highly malignant neuroblastoma cells to host immune response. © 2001 Wiley-Liss, Inc.

Publication metadata

Author(s): Annicchiarico-Petruzzelli M, Bernassola F, Lovat PE, Redfern CPF, Pearson ADJ, Melino G

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: 8th Advances in Neuroblastoma Research Meeting

Year of Conference: 1998

Pages: 115-117

ISSN: 1545-5009

Publisher: Medical and Pediatric Oncology: John Wiley & Sons, Inc.


DOI: 10.1002/1096-911X(20010101)36:1<115::AID-MPO1027>3.0.CO;2-X

PubMed id: 11464860