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Analysis of intact bacteriohopanepolyols from methanotrophic bacteria by reversed-phase high-performance liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry

Lookup NU author(s): Dr Helen Talbot, Diane Watson, Dr Paul Farrimond


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Direct detection of most intact biohopanoids is not possible using conventional GC-MS techniques due to their highly functionalised and amphiphilic nature. Here we report the application of a new reversed-phase high-performance liquid chromatography method for the direct analysis of acetylated, intact bacteriohopanepolyols in solvent extracts of methanotrophic bacteria. Atmospheric pressure chemical ionisation mass spectrometric detection provides structural information relating to the number and types of functional groups present in the four biohopanoids detected: bacteriohopanetetrol, aminobacteriohopanetriol, -tetrol and -pentol. The method should facilitate the assessment of hopanoid composition of both bacteria and environmental samples. © 2001 Elsevier Science B.V.

Publication metadata

Author(s): Talbot HM, Watson D, Murrell J, Carter J, Farrimond P

Publication type: Article

Publication status: Published

Journal: Journal of Chromatography A

Year: 2001

Volume: 921

Issue: 2

Pages: 175-185

ISSN (print): 0021-9673

ISSN (electronic): 1873-3778

Publisher: Elsevier BV


DOI: 10.1016/S0021-9673(01)00871-8

PubMed id: 11471801


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