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Lookup NU author(s): Dr Helen Talbot,
Dr Paul Farrimond
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Direct detection of most intact biohopanoids is not possible using conventional GC-MS techniques due to their highly functionalised and amphiphilic nature. Here we report the application of a new reversed-phase high-performance liquid chromatography method for the direct analysis of acetylated, intact bacteriohopanepolyols in solvent extracts of methanotrophic bacteria. Atmospheric pressure chemical ionisation mass spectrometric detection provides structural information relating to the number and types of functional groups present in the four biohopanoids detected: bacteriohopanetetrol, aminobacteriohopanetriol, -tetrol and -pentol. The method should facilitate the assessment of hopanoid composition of both bacteria and environmental samples. © 2001 Elsevier Science B.V.
Author(s): Talbot HM, Watson D, Murrell J, Carter J, Farrimond P
Publication type: Article
Publication status: Published
Journal: Journal of Chromatography A
ISSN (print): 0021-9673
ISSN (electronic): 1873-3778
Publisher: Elsevier BV
PubMed id: 11471801
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