Toggle Main Menu Toggle Search

Open Access padlockePrints

The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel

Lookup NU author(s): Dr Michael Gray


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties. © 2004 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Sheppard DN, Gray MA, Gong X, Sohma Y, Kogan I, Benos DJ, Scott-Ward TS, Chen J-H, Li H, Cai Z, Gupta J, Li C, Ramjeesingh M, Berdiev BK, Ismailov II, Bear CE, Hwang T-C, Linsdell P, Hug MJ

Publication type: Article

Publication status: Published

Journal: Journal of Cystic Fibrosis

Year: 2004

Volume: 3

Issue: Suppl. 2

Pages: 101-108

ISSN (print): 1569-1993

ISSN (electronic): 1873-5010

Publisher: Elsevier BV


DOI: 10.1016/j.jcf.2004.05.046

PubMed id: 15463939


Altmetrics provided by Altmetric