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EXO1 Plays a Role in Generating Type I and Type II Survivors in Budding Yeast

Lookup NU author(s): Dr Laura Maringele, Professor David Lydall


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Telomerase-defective budding yeast cells escape senescence by using homologous recombination to amplify telomeric or subtelomeric structures. Similarly, human cells that enter senescence can use homologous recombination for telomere maintenance, when telomerase cannot be activated. Although recombination proteins required to generate telomerase-independent survivors have been intensively studied, little is known about the nucleases that generate the substrates for recombination. Here we demonstrate that the Exo1 exonuclease is an initiator of the recombination process that allows cells to escape senescence and become immortal in the absence of telomerase. We show that EXO1 is important for generating type I survivors in yku70Δ mre11Δ cells and type II survivors in tlc1Δ cells. Moreover, in tlc1Δ cells, EXO1 seems to contribute to the senescence process itself.

Publication metadata

Author(s): Maringele L, Lydall D

Publication type: Article

Publication status: Published

Journal: Genetics

Year: 2004

Volume: 166

Issue: 4

Pages: 1641-1649

ISSN (print): 0016-6731

ISSN (electronic): 1943-2631

Publisher: Genetics Society of America


DOI: 10.1534/genetics.166.4.1641

PubMed id: 15126386


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