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Lookup NU author(s): Dr Nick Watkins
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In the nucleolus the U3 snoRNA is recruited to the 80S pre-rRNA processing complex in the dense fibrillar component (DFC). The U3 snoRNA is found throughout the nucleolus and has been proposed to move with the preribosomes to the granular component (GC). In contrast, the localization of other RNAs, such as the U8 snoRNA, is restricted to the DFC. Here we show that the incorporation of the U3 snoRNA into the 80S processing complex is not dependent on pre-rRNA base pairing sequences but requires the B/C motif, a U3-specific protein-binding element. We also show that the binding of Mpp10 to the 80S U3 complex is dependent on sequences within the U3 snoRNA that base pair with the pre-rRNA adjacent to the initial cleavage site. Furthermore, mutations that inhibit 80S complex formation and/or the association of Mpp10 result in retention of the U3 snoRNA in the DFC. From this we propose that the GC localization of the U3 snoRNA is a direct result of its active involvement in the initial steps of ribosome biogenesis.
Author(s): Granneman S, Vogelzangs J, Luhrmann R, Van Venrooij WJ, Pruijn GJM, Watkins NJ
Publication type: Article
Publication status: Published
Journal: Molecular and Cellular Biology
ISSN (print): 0270-7306
ISSN (electronic): 1067-8824
Publisher: American Society for Microbiology
PubMed id: 15367679
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