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Lookup NU author(s): Dr Michael Gray, Professor Barry Argent
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We describe an air-liquid interface primary culture method for murine tracheal epithelial cells on semi-permeable membranes, forming polarized epithelia with a high transepithelial resistance, differentiation to ciliated and secretory cells, and physiologically appropriate expression of key genes and ion channels. We also describe the isolation of primary murine nasal epithelial cells for patch-clamp analysis, generating polarised cells with physiologically appropriate distribution and ion channel expression. These methods enable more physiologically relevant analysis of murine airway epithelial cells in vitro and ex vivo, better utilisation of transgenic mouse models of human pulmonary diseases, and have been approved by the European Working Group on CFTR expression. © 2004 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
Author(s): Davidson DJ, Gray MA, Kilanowski FM, Tarran R, Randell SH, Sheppard DN, Argent BE, Dorin JR
Publication type: Article
Publication status: Published
Journal: Journal of Cystic Fibrosis
Year: 2004
Volume: 3
Issue: 2
Pages: 59-62
ISSN (print): 1569-1993
ISSN (electronic): 1873-5010
Publisher: Elsevier BV
URL: http://dx.doi.org/10.1016/j.jcf.2004.05.013
DOI: 10.1016/j.jcf.2004.05.013
PubMed id: 15463928
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