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The use of a 3895 bp mitochondrial DNA deletion as a marker for sunlight exposure in human skin

Lookup NU author(s): Kim Krishnan, Dr Andrew Harbottle, Professor Mark Birch-Machin

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Abstract

Previous work has examined the use of mitochondrial DNA (mtDNA) damage as a biomarker of cumulative sun exposure in human skin. These studies have simply compared mtDNA damage between sun-protected and sun exposed skin. This approach is limited because non-melanoma skin cancer (NMSC) is predominantly formed on body sites which are 'usually' sun exposed as opposed to sites which are Occasionally' sun exposed and as such they differ in their cumulative ultraviolet (UV) exposure. This study addresses this limitation by investigating the frequency of occurrence of a rarely reported 3895 bp mtDNA deletion in 104 age-matched human skin samples taken from different sun-exposed body sites. There was a significant increase in the deletion frequency with increasing UV exposure (p < 0.0001). Furthermore there was a significantly greater deletion frequency in 'usually' sun exposed compared with 'occasionally' sun-exposed body sites in both the dermis (p = 0.0018) and epidermis (p < 0.0001). Investigation of the 3895 bp deletion in the same NMSC samples used in a previous study of the 4977 bp common deletion, showed a greater frequency of the 3895 bp deletion (8/10 vs 4/10, respectively). Additionally, we have linked the 3895 bp deletion with the UVR component of sunlight by inducing the deletion in vitro with repetitive sub-lethal doses of a UVA+UVB light source.


Publication metadata

Author(s): Krishnan KJ, Harbottle A, Birch-Machin MA

Publication type: Article

Publication status: Published

Journal: Journal of Investigative Dermatology

Year: 2004

Volume: 123

Issue: 6

Pages: 1020-1024

ISSN (print): 0022-202X

ISSN (electronic): 1523-1747

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1111/j.0022-202X.2004.23457.x

DOI: 10.1111/j.0022-202X.2004.23457.x

PubMed id: 15610508


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