Toggle Main Menu Toggle Search

Open Access padlockePrints

UT-B is expressed in bovine rumen: Potential role in ruminal urea transport

Lookup NU author(s): Dr Gavin Stewart, Dr Christopher Graham, Professor Nicholas Simmons

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

The UT-A (SLC14a2) and UT-B (SLC14a1) genes encode a family of specialized urea transporter proteins that regulate urea movement across plasma membranes. In this report, we describe the structure of the bovine UT-B (bUT-B) gene and characterize UT-B expression in bovine rumen. Northern analysis using a full-length bUT-B probe detected a 3.7-kb UT-B signal in rumen. RT-PCR of bovine mRNA revealed the presence of two UT-B splice variants, bUT-B1 and bUT-B2, with bUT-B2 the predominant variant in rumen. Immunoblotting studies of bovine rumen tissue, using an antibody targeted to the NH2-terminus of mouse UT-B, confirmed the presence of 43- to 54-kDa UT-B proteins. Immunolocalization studies showed that UT-B was mainly located on cell plasma membranes in epithelial layers of the bovine rumen. Using chamber measurements of ruminal transepithelial transport of 14C-labeled urea indicated that urea flux was characteristically inhibited by phloretin. We conclude that bUT-B is expressed in the bovine rumen and may function to transport urea into the rumen as part of the ruminant urea nitrogen salvaging process.


Publication metadata

Author(s): Stewart GS, Graham C, Cattell S, Smith TPL, Simmons NL, Smith CP

Publication type: Article

Publication status: Published

Journal: American Journal of Physiology - Regulatory Integrative and Comparative Physiology

Year: 2005

Volume: 289

Issue: 2

Pages: R605-R612

Print publication date: 01/08/2005

ISSN (print): 0363-6119

ISSN (electronic): 1522-1490

Publisher: American Physiological Society

URL: http://dx.doi.org/10.1152/ajpregu.00127.2005

DOI: 10.1152/ajpregu.00127.2005

PubMed id: 15845882


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share