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Human-serum matrix supports undifferentiated growth of human embryonic stem cells

Lookup NU author(s): Petra Stojkovic, Professor Majlinda LakoORCiD, Professor Lyle Armstrong, Jerome Evans, Professor Miodrag Stojkovic


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One of the most frequently used matrices for feeder-free growth of undifferentiated human embryonic stem cells (hESCs) is Matrigel, which supports attachment and growth of undifferentiated hESCs in the presence of mouse embryonic fibroblast-conditioned medium. Unfortunately, application of Matrigel or medium conditioned by mouse embryonic feeder cells is not ideal for potential medical application of hESCs because xenogeneic pathogens can be transmitted through culture conditions. We demonstrate here that human serum as matrix and medium conditioned by differentiated hESCs reduce exposure of hESCs to animal ingredients and provide a safer direction toward completely animal-free conditions for application, handling, and understanding of hESC biology. At thesametime, hESCs grown under these conditions maintain all hESC features after prolonged culture, including the developmental potential to differentiate into representative tissues of all three embryonic germ layers, unlimited and undifferentiated proliferative ability, and maintenance of normalkaryotype. ©AlphaMed Press.

Publication metadata

Author(s): Stojkovic P, Lako M, Przyborski S, Stewart R, Armstrong L, Evans J, Zhang X, Stojkovic M

Publication type: Article

Publication status: Published

Journal: Stem Cells

Year: 2005

Volume: 23

Issue: 7

Pages: 895-902

Print publication date: 01/08/2005

ISSN (print): 1066-5099

ISSN (electronic): 1549-4918

Publisher: AlphaMed Press, Inc.


DOI: 10.1634/stemcells.2004-0326

PubMed id: 15888688


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Funder referenceFunder name
BBS/B/14779Biotechnology and Biological Sciences Research Council
G0301182Medical Research Council