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3D modelling, gene expression mapping and post-mapping image analysis in the developing human brain

Lookup NU author(s): Dr Subrot Sarma, Dr Janet Kerwin, Mark Scott, Professor Tom Strachan, Emerita Professor Susan Lindsay


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As human brain development proceeds, there are complex changes in size and shape, most notably in the developing forebrain. Molecular technologies enable us to characterise the gene expression patterns that underlie these changes. To interpret these patterns the location of expression must be identified and, often, gene expression patterns compared for several genes or across several developmental stages. To facilitate interpretation we have generated a set of three-dimensional models using a recently developed technique, optical projection tomography. The models act as a framework onto which gene expression patterns are mapped and anatomical domains identified using custom-designed software, MAPaint. Here, we demonstrate their use to compare forebrain development at two embryonic stages (Carnegie stages 18 and 21; 44 and 52 days post conception, respectively) and as a means of recording, storing and visualising gene expression data for three example genes EMX1, EMX2 and OTX2. Anatomical domains were also mapped to the models and the comparison of gene expression and anatomical data is demonstrated at Carnegie stage 21. The three-dimensional models and sophisticated software facilitate the analysis and visualisation of morphological changes and gene expression patterns during early brain development and can be applied to the development of other complex structures. © 2005 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Sarma S, Kerwin J, Puelles L, Scott M, Strachan T, Feng G, Sharpe J, Davidson D, Baldock R, Lindsay S


Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Evolution and Development of Nervous Systems - Proceedings of the 4th European Conference on Comparative Neurobiology

Year of Conference: 2005

Pages: 449-453

ISSN: 0361-9230

Publisher: Brain Research Bulletin, Elsevier


DOI: 10.1016/j.brainresbull.2005.05.022

PubMed id: 16144630

Library holdings: Search Newcastle University Library for this item

ISBN: 18732747