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Lookup NU author(s): David Nury, Dr Herve Chabanon, Professor John Hesketh
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Localization of mRNAs provides a novel mechanism for synthesis of proteins close to their site of function. MT1 (metallothionein-1) is a small, metal-binding protein that is largely cytoplasmic but which can be found in the nucleus. The localization of rat MT1 requires the perinuclear localization of its mRNA by a mechanism dependent on the 3′-UTR (3′-untranslated region). The present study investigates the nature of this mRNA localization signal using Chinese-hamster ovary cells transfected with gene constructs in which either MT1 or the globin coding region is linked to different sequences from the MT1 3′-UTR. Deletion, mutagenesis and antisense oligonucleotide approaches indicate that nt 45-76 of the 3′-UTR, in particular nt 66-76, are required for the localization of either MT1 mRNA or chimaeric transcripts in which a β-globin coding region is linked to sequences from the MT1 3′-UTR. This section of the 3′-UTR contains a CACC repeat. Two mutations that are predicted to alter the secondary structure of this region also impair localization. Our hypothesis is that the perinuclear localization signal in MT1 mRNA is formed by a combination of the CACC repeat and its structural context. © 2005 Biochemical Society.
Author(s): Nury D, Chabanon H, Levadoux-Martin M, Hesketh J
Publication type: Article
Publication status: Published
Journal: Biochemical Journal
Year: 2005
Volume: 387
Issue: 2
Pages: 419-428
Print publication date: 15/04/2005
ISSN (print): 0264-6021
ISSN (electronic): 1470-8728
Publisher: Biochemical Society
URL: http://dx.doi.org/10.1042/BJ20040630
DOI: 10.1042/BJ20040630
PubMed id: 15537387
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