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Perinuclear localisation of cellular retinoic acid binding protein I mRNA

Lookup NU author(s): Adrian Blackburn, Dr Herve Chabanon, Professor John Hesketh

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Abstract

Retinoids are important metabolic and developmental regulators that act through nuclear receptors. The cellular retinoic acid binding protein CRABPI has been suggested to play a role in trafficking of retinoic acid but its exact functions and subcellular localisation remain unclear. Here we show that in CHO cells both exogenous CRABPI transcripts and tagged CRABPI protein have a perinuclear distribution that depends upon the 3′UTR of the mRNA. The CRABPI 3′UTR conferred perinuclear localisation on globin reporter transcripts. Deletion analysis indicated that the first 123nt of CRABPI 3′UTR are necessary for localisation of both CRABPI mRNA and protein. We propose that CRABPI mRNA is localised by a signal within its 3′UTR and that this partly determines the distribution of CRABPI protein. © 2005 Elsevier Inc. All rights reserved.


Publication metadata

Author(s): Levadoux-Martin M, Li Y, Blackburn A, Chabanon H, Hesketh JE

Publication type: Article

Publication status: Published

Journal: Biochemical and Biophysical Research Communications

Year: 2006

Volume: 340

Issue: 1

Pages: 326-331

ISSN (print): 0006-291X

ISSN (electronic): 1090-2104

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.bbrc.2005.12.006

DOI: 10.1016/j.bbrc.2005.12.006

PubMed id: 16376305


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