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In vitro biofilm formation on the surface of resin-based dentine adhesives

Lookup NU author(s): Dr Sarah Rolland, Professor John McCabe, Professor Angus Walls


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Prevention of root caries on exposed root surfaces in the aging population is a significant challenge. Bonding resins can be applied to exposed root surfaces as sealants; however, minimal data exists regarding biofilm formation on the surface of these resins. We hypothesized that an antibacterial dentine-bonding resin containing methacryloxydodecyl-pyridiniumbromide (MDPB) may reduce biofilm formation. Biofilms were produced in pooled stimulated natural whole saliva, supplemented with 1% sucrose, on the surface of 5 dentine-bonding resins (Clearfil SE, OptiBond Solo, Protect Bond, Protect Bond Primer, and Xeno III) using untreated root surfaces as controls. Biofilms were stained using the Live:Dead Baclight bacterial viability stain, viewed with confocal microscopy, and analyzed using ImageJ image-analysis software. Resin surfaces encouraged attachment of live bacteria compared with root surfaces. All resins showed similar bacterial colonization in sections adjacent to the resin surface, but in the central and outer portions of biofilms, Xeno III and Protect Bond Primer showed a viable bacterial load similar to that of the root surface. Fluoride-releasing resins (OptiBond Solo/Protect Bond) did not show reduced biofilm formation. Thus, antibacterial agents within the resins have a minimal effect on biofilm formation, particularly when directly adjacent to the root surface. © 2006 The Authors.

Publication metadata

Author(s): Rolland SL, McCabe JF, Robinson C, Walls AWG

Publication type: Article

Publication status: Published

Journal: European Journal of Oral Sciences

Year: 2006

Volume: 114

Issue: 3

Pages: 243-249

ISSN (print): 0909-8836

ISSN (electronic): 1600-0722

Publisher: Wiley-Blackwell


DOI: 10.1111/j.1600-0722.2006.00359.x

PubMed id: 16776774


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