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A novel electrode-pipette design for simultaneous recording of extracellular spikes and iontophoretic drug application in awake behaving monkeys

Lookup NU author(s): Professor Alexander Thiele, Dr Louise Delicato, Dr Mark Roberts, Dr Alwin GieselmannORCiD


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We developed a novel design of an electrode-pipette combination (EPC) which allows access to brain structures in awake behaving primates without the need for guide tubes or to mechanically open the dura prior to electrode insertion. The EPC consists of an etched tungsten in glass electrode flanked by two pipettes which allow for local and highly controlled iontophoretic administration of neuroactive substances. These EPCs have excellent single cell isolation properties and are sturdy enough to penetrate the primate dura for up to 8 weeks following either a craniotomy or a dura scrape (i.e. even after substantial built up of fibrous scar tissue). We show that the EPCs can be used to selectively manipulate the cholinergic system in primate V1 during passive fixation and while animals perform an attentionally demanding task. © 2006 Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Thiele A, Delicato LS, Roberts MJ, Gieselmann MA

Publication type: Article

Publication status: Published

Journal: Journal of Neuroscience Methods

Year: 2006

Volume: 158

Issue: 2

Pages: 207-211

ISSN (print): 0165-0270

ISSN (electronic): 1872-678X

Publisher: Elsevier BV


DOI: 10.1016/j.jneumeth.2006.05.032

PubMed id: 16843532


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Funder referenceFunder name
070380/Z/03/ZWellcome Trust
070380Wellcome Trust
BBS/B/09325Biotechnology and Biological Sciences Research Council