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Comparison of three multiplex cytokine analysis systems: Luminex, SearchLight™ and FAST Quant®

Lookup NU author(s): Dr Gendie Lash, Barbara Innes, Dr Harry Otun, Professor Steve RobsonORCiD, Dr Roger Searle, Dr Judith Bulmer

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Abstract

Multiplex cytokine analysis technologies have become readily available in the last five years. Two main formats exist: multiplex sandwich ELISA and bead based assays. While these have each been compared to individual ELISAs, there has been no direct comparison between the two formats. We report here the comparison of two multiplex sandwich ELISA procedures (FAST Quant and SearchLight) and a bead based assay (UpState Luminex). All three kits differed from each other for different analytes and there was no clear pattern of one system giving systematically different results than another for any analyte studied. We suggest that each system has merits and several factors including range of analytes available, prospect of development of new analytes, dynamic range of the assay, sensitivity of the assay, cost of equipment, cost of consumables, ease of use and ease of data analysis need to be considered when choosing a system for use. We also suggest that results obtained from different systems cannot be combined. © 2006 Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Lash GE, Scaife PJ, Innes BA, Otun HA, Robson SC, Searle RF, Bulmer JN

Publication type: Article

Publication status: Published

Journal: Journal of Immunological Methods

Year: 2006

Volume: 309

Issue: 1-2

Pages: 205-208

ISSN (print): 0022-1759

ISSN (electronic): 1872-7905

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/j.jim.2005.12.007

DOI: 10.1016/j.jim.2005.12.007

PubMed id: 16436279

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