Browse by author
Lookup NU author(s): Julie Birraux, Emeritus Professor John Kirby, Professor Mark Thomason, Dr John TaylorORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Background and Objective: Gingival overgrowth (GO) is a side-effect of cyclosporin A (CsA) therapy and is characterised by enlargement of the gingiva with epithelial thickening and overproduction of extracellular matrix components. The pathogenesis of the epithelial thickening in GO remains obscure. The objective of the present study was to investigate the effects of CsA on the growth of oral epithelial cells in vitro and to test the hypothesis that CsA influences apoptosis in these cells. Material and Methods: Cyclosporin was cocultured with an immortalized normal human oral keratinocyte cell line (HOK-16B), an epitheloid cervical carcinoma cell line (HeLa) and primary oral keratinocytes. Cell division was quantified using a CyQUANT™ kit. Apoptosis was induced using tumour necrosis factor-α (TNF-α) and assayed by analysis of caspase-3 activity. Expression of the anti-apoptotic protein, Bcl-2, was measured by western blotting. Results: CsA exhibited a dose- and time-dependent inhibition of cell division in all three keratinocyte cell cultures. Significantly, HOK-16B cells treated with high doses of CsA (10 μg/ml) did not recover their proliferative capacity 3 d after withdrawal of CsA, indicating that CsA-induced inhibition of growth is not temporary. Concentrations of CsA that inhibited cell division (1 μg/ml) did not have any effect on constitutive or TNF-α -induced apoptosis or Bcl-2 expression in HOK-16B cells. Conclusion: CsA inhibits oral epithelial cell division and this effect is not associated with changes in apoptosis in these cells. The action of CsA on oral epithelial cells may be associated with a long-lasting stress signal, which might account for some of the pathological effects of this drug. © 2006 Blackwell Munksgaard.
Author(s): Birraux J, Kirby JA, Thomason JM, Taylor JJ
Publication type: Article
Publication status: Published
Journal: Journal of Periodontal Research
Year: 2006
Volume: 41
Issue: 4
Pages: 297-302
Print publication date: 01/08/2006
ISSN (print): 0022-3484
ISSN (electronic): 1600-0765
Publisher: Wiley-Blackwell Munksgaard
URL: http://dx.doi.org/10.1111/j.1600-0765.2006.00873.x
DOI: 10.1111/j.1600-0765.2006.00873.x
PubMed id: 16827723
Altmetrics provided by Altmetric
