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Explaining isotope trophic-step fractionation: Why herbivorous fish are different

Lookup NU author(s): Professor Aileen MillORCiD, John Pinnegar, Professor Nick Polunin


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1. An assumed constant trophic fractionation of 15N/ 14N between consumer and diet (usually 3.4‰ for diet-muscle tissue differences) allows inferences to be made about feeding interactions and trophic level in food web studies. However, considerable variability surrounds this constant, which may conceal subtle differences about the trophodynamics of consumers. 2. The feeding ecologies of herbivores and carnivores differ in terms of diet quality (in C : N terms) and food processing mechanisms, which may affect fractionation. 3. We present a new model that explores how consumer feeding rates, excretion rates and diet quality determine the 15N/14N ratios in the consumer's tissues and hence influence the magnitude of trophic fractionation. 4. Three herbivorous reef fish Acanthurus sohal, Zebrasoma xanthurum and Pomacentrus arabicus were chosen as study organisms. Empirical estimates of diet-tissue stable isotope fractionation were made in the field, and model parameters were derived from feeding observations and literature data. 5. The trophic fractionation values of A. sohal, Z. xanthurum and P. arabicus were 4.69, 4.47 and 5.25, respectively, by empirical measurement, and 4.41, 4.30 and 5.68, respectively, by model, indicating that herbivores have a higher trophic fractionation than the currently accepted value of 3.4. 6. The model was most sensitive to the excretion rate, which may differ between herbivores and carnivorous animals. This model is the first to determine stable isotope signatures of a consumer's diet mixture without applying a constant fractionation value. © 2007 The Authors.

Publication metadata

Author(s): Mill AC, Pinnegar JK, Polunin NVC

Publication type: Article

Publication status: Published

Journal: Functional Ecology

Year: 2007

Volume: 21

Issue: 6

Pages: 1137-1145

ISSN (print): 0269-8463

ISSN (electronic): 1365-2435

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1111/j.1365-2435.2007.01330.x


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